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Articles (71)
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From cryo-EM, multiple protein structures in one shot

Sigworth, Fred J

Journal:

Nature Methods

Année:

2007

Langue:

english

Fichier:

PDF, 154 KB

english, 2007

Spherical Reconstruction: A Method for Structure Determination of Membrane Proteins from Cryo-EM Images

Qiu-Xing Jiang, David W. Chester, Fred J. Sigworth

Journal:

Journal of Structural Biology

Année:

2001

Langue:

english

Fichier:

PDF, 540 KB

english, 2001

Importance of the Debye Screening Length on Nanowire Field Effect Transistor Sensors

Stern, Eric, Wagner, Robin, Sigworth, Fred J., Breaker, Ronald, Fahmy, Tarek M., Reed, Mark A.

Journal:

Nano Letters

Année:

2007

Langue:

english

Fichier:

PDF, 177 KB

english, 2007

[IEEE 2008 IEEE International Symposium on Circuits and Systems - ISCAS 2008 - Seattle, WA, USA (2008.05.18-2008.05.21)] 2008 IEEE International Symposium on Circuits and Systems - An integrated patch-clamp amplifier for high-throughput planar patch-clamp systems

Weerakoon, Pujitha, Culurciello, Eugenio, Klemic, Kate, Sigworth, Fred J.

Année:

2008

Langue:

english

Fichier:

PDF, 671 KB

english, 2008

[IEEE 2010 IEEE International Symposium on Circuits and Systems - ISCAS 2010 - Paris, France (2010.05.30-2010.06.2)] Proceedings of 2010 IEEE International Symposium on Circuits and Systems - An integrated patch-clamp system with dual Input

Weerakoon, Pujitha, Sigworth, Fred, Kindlmann, Peter, Santos-Sacchi, Joseph, Yang, Youshan, Culurciello, Eugenio

Année:

2010

Langue:

english

Fichier:

PDF, 381 KB

english, 2010

[IEEE 2007 IEEE International Symposium on Circuits and Systems - New Orleans, LA, USA (2007.05.27-2007.05.30)] 2007 IEEE International Symposium on Circuits and Systems - An Integrated Patch-Clamp Amplifier for High-Density Whole-Cell Recordings

Weerakoon, Pujitha, Klemic, Kate, Sigworth, Fred J., Culurciello, Eugenio

Année:

2007

Langue:

english

Fichier:

PDF, 403 KB

english, 2007

Removal of Vesicle Structures From Transmission Electron Microscope Images

Jensen, Katrine Hommelhoff, Sigworth, Fred J., Brandt, Sami Sebastian

Journal:

IEEE Transactions on Image Processing

Année:

2016

Langue:

english

Fichier:

PDF, 9.47 MB

english, 2016

Statistical modeling and removal of lipid membrane projections for cryo-EM structure determination of reconstituted membrane proteins

Jensen, Katrine Hommelhoff, Brandt, Sami Sebastian, Shigematsu, Hideki, Sigworth, Fred J.

Journal:

Journal of Structural Biology

Année:

2016

Langue:

english

Fichier:

PDF, 4.40 MB

english, 2016

Synthetic Protocells to Mimic and Test Cell Function

Jian Xu, Fred J. Sigworth, David A. LaVan

Journal:

Advanced Materials

Année:

2010

Langue:

english

Fichier:

PDF, 487 KB

english, 2010

Reference free structure determination through eigenvectors of center of mass operators

Ronald R. Coifman, Yoel Shkolnisky, Fred J. Sigworth, Amit Singer

Journal:

Applied and Computational Harmonic Analysis

Année:

2010

Langue:

english

Fichier:

PDF, 2.61 MB

english, 2010

Patch-clamp amplifiers on a chip

Pujitha Weerakoon, Eugenio Culurciello, Youshan Yang, Joseph Santos-Sacchi, Peter J. Kindlmann, Fred J. Sigworth

Journal:

Journal of Neuroscience Methods

Année:

2010

Langue:

english

Fichier:

PDF, 1.08 MB

english, 2010

Holey carbon micro-arrays for transmission electron microscopy: A microcontact printing approach

David W. Chester, James F. Klemic, Eric Stern, Fred J. Sigworth, Kathryn G. Klemic

Journal:

Ultramicroscopy

Année:

2007

Langue:

english

Fichier:

PDF, 1.19 MB

english, 2007

Micromolded PDMS planar electrode allows patch clamp electrical recordings from cells

Kathryn G. Klemic, James F. Klemic, Mark A. Reed, Fred J. Sigworth

Journal:

Biosensors and Bioelectronics

Année:

2002

Langue:

english

Fichier:

PDF, 439 KB

english, 2002

An air-molding technique for fabricating PDMS planar patch-clamp electrodes

Kathryn G. Klemic, James F. Klemic, Fred J. Sigworth

Journal:

Pflügers Archiv European Journal of Physiology

Année:

2005

Langue:

english

Fichier:

PDF, 418 KB

english, 2005

Structural biology: Life's transistors

Sigworth, Fred J.

Journal:

Nature

Année:

2003

Langue:

english

Fichier:

PDF, 109 KB

english, 2003

Structure of the BK potassium channel in a lipid membrane from electron cryomicroscopy

Wang, Liguo, Sigworth, Fred J.

Journal:

Nature

Année:

2009

Langue:

english

Fichier:

PDF, 700 KB

english, 2009

Fragile X mental retardation protein controls gating of the sodium-activated potassium channel Slack

Brown, Maile R, Kronengold, Jack, Gazula, Valeswara-Rao, Chen, Yi, Strumbos, John G, Sigworth, Fred J, Navaratnam, Dhasakumar, Kaczmarek, Leonard K

Journal:

Nature Neuroscience

Année:

2010

Langue:

english

Fichier:

PDF, 471 KB

english, 2010

Two conformational states of the membrane-associated Bacillus thuringiensis Cry4Ba δ-endotoxin complex revealed by electron crystallography: Implications for toxin-pore formation

Puey Ounjai, Vinzenz M. Unger, Fred J. Sigworth, Chanan Angsuthanasombat

Journal:

Biochemical and Biophysical Research Communications

Année:

2007

Langue:

english

Fichier:

PDF, 982 KB

english, 2007

Classical detection theory and the cryo-EM particle selection problem

Fred J. Sigworth

Journal:

Journal of Structural Biology

Année:

2004

Langue:

english

Fichier:

PDF, 624 KB

english, 2004

Software tools for macromolecular microscopy

Bridget Carragher, Clinton S. Potter, Fred J. Sigworth

Journal:

Journal of Structural Biology

Année:

2007

Langue:

english

Fichier:

PDF, 75 KB

english, 2007

Streptavidin crystals as nanostructured supports and image-calibration references for cryo-EM data collection

Liguo Wang, Puey Ounjai, Fred J. Sigworth

Journal:

Journal of Structural Biology

Année:

2008

Langue:

english

Fichier:

PDF, 905 KB

english, 2008

Detecting consistent common lines in cryo-EM by voting

Amit Singer, Ronald R. Coifman, Fred J. Sigworth, David W. Chester, Yoel Shkolnisky

Journal:

Journal of Structural Biology

Année:

2010

Langue:

english

Fichier:

PDF, 928 KB

english, 2010

An adaptive Expectation–Maximization algorithm with GPU implementation for electron cryomicroscopy

Hemant D. Tagare, Andrew Barthel, Fred J. Sigworth

Journal:

Journal of Structural Biology

Année:

2010

Langue:

english

Fichier:

PDF, 1.08 MB

english, 2010

A Bayesian adaptive basis algorithm for single particle reconstruction

Alp Kucukelbir, Fred J. Sigworth, Hemant D. Tagare

Journal:

Journal of Structural Biology

Année:

2012

Langue:

english

Fichier:

PDF, 1.18 MB

english, 2012

Pharmacological activation and inhibition of Slack (Slo2.2) channels

Bo Yang, Valentin K. Gribkoff, Jennifer Pan, Veronique Damagnez, Steven I. Dworetzky, Christopher G. Boissard, Arin Bhattacharjee, Yangyang Yan, Fred J. Sigworth, Leonard K. Kaczmarek

Journal:

Neuropharmacology

Année:

2006

Langue:

english

Fichier:

PDF, 649 KB

english, 2006

A novel conotoxin, qc16a, with a unique cysteine framework and folding

Mingyu Ye, Jing Hong, Mi Zhou, Lijun Huang, Xiaoxia Shao, Youshan Yang, Fred J. Sigworth, Chengwu Chi, Donghai Lin, Chunguang Wang

Journal:

Peptides

Année:

2011

Langue:

english

Fichier:

PDF, 465 KB

english, 2011

Potassium Channel Mechanics

Fred J Sigworth

Journal:

Neuron

Année:

2001

Langue:

english

Fichier:

PDF, 32 KB

english, 2001

Hydration-layer models for cryo-EM image simulation

Zhiguo Shang, Fred J. Sigworth

Journal:

Journal of Structural Biology

Année:

2012

Langue:

english

Fichier:

PDF, 1.31 MB

english, 2012

Patch Clamp on a Chip

Sigworth, Fred J., Klemic, Kathryn G.

Journal:

Biophysical Journal

Année:

2002

Langue:

english

Fichier:

PDF, 47 KB

english, 2002

Improved Hidden Markov Models for Molecular Motors, Part 2: Extensions and Application to Experimental Data

Syed, Sheyum, Müllner, Fiona E., Selvin, Paul R., Sigworth, Fred J.

Journal:

Biophysical Journal

Année:

2010

Langue:

english

Fichier:

PDF, 496 KB

english, 2010

Improved Hidden Markov Models for Molecular Motors, Part 1: Basic Theory

Müllner, Fiona E., Syed, Sheyum, Selvin, Paul R., Sigworth, Fred J.

Journal:

Biophysical Journal

Année:

2010

Langue:

english

Fichier:

PDF, 801 KB

english, 2010

The Last Few Frames of the Voltage-Gating Movie

Sigworth, Fred J.

Journal:

Biophysical Journal

Année:

2007

Langue:

english

Fichier:

PDF, 57 KB

english, 2007

[Methods in Enzymology] Cryo-EM, Part B: 3-D Reconstruction Volume 482 || An Introduction to Maximum-Likelihood Methods in Cryo-EM

Sigworth, Fred J.

Année:

2010

Langue:

english

Fichier:

PDF, 565 KB

english, 2010

Expression, Purification and Functional Reconstitution of Slack Sodium-Activated Potassium Channels

Yan, Yangyang, Yang, Youshan, Bian, Shumin, Sigworth, Fred J.

Journal:

Journal of Membrane Biology

Année:

2012

Langue:

english

Fichier:

PDF, 502 KB

english, 2012

Slack Channels are Sensitive to External Sodium

Yang, Youshan, Yan, Yangyang, Sigworth, Fred J.

Journal:

Biophysical Journal

Année:

2012

Langue:

english

Fichier:

PDF, 39 KB

english, 2012

State-Dependent FRET Reports Large Gating-Ring Motions in BK Channels

Miranda, Pablo, Contreras, Jorge E., Wesch, Diana, Sigworth, Fred J., Holmgren, Miguel, Giraldez, Teresa

Journal:

Biophysical Journal

Année:

2012

Langue:

english

Fichier:

PDF, 41 KB

english, 2012

Identification of Regulatory Phosphorylation Sites in Slack and Slick Sodium Activated Potassium (KNa) Channels

Fleming, Matthew R., Kronengold, Jack, Yan, Yangyang, Lam, Tukiet T., Gulcicek, Erol E., Sigworth, Fred J., Nairn, Angus C., Kaczmarek, Leonard K.

Journal:

Biophysical Journal

Année:

2010

Langue:

english

Fichier:

PDF, 46 KB

english, 2010

Automatic cryo-EM particle selection for membrane proteins in spherical liposomes

Liu, Yunhui, Sigworth, Fred J.

Journal:

Journal of Structural Biology

Année:

2014

Langue:

english

Fichier:

PDF, 2.46 MB

english, 2014

Functional Tests Of Purified Slack Channel Protein For Cryo-em Structure Determination

Yang, Youshan, Yan, Yangyang, Sigworth, Fred J.

Journal:

Biophysical Journal

Année:

2009

Langue:

english

Fichier:

PDF, 89 KB

english, 2009

Expression and Purification of the BK Channel Alpha-Beta1 Complex

Zhang, Yixuan, Yan, Yangyang, Yang, Youshan, Sigworth, Fred

Journal:

Biophysical Journal

Année:

2011

Langue:

english

Fichier:

PDF, 36 KB

english, 2011

Measuring Membrane Potentials with Cryo-EM?

Chen, Yi, Shigematsu, Hideki, Nagayama, Kuniaki, Sigworth, Fred J.

Journal:

Biophysical Journal

Année:

2012

Langue:

english

Fichier:

PDF, 40 KB

english, 2012

Calcium Induces Structural Rearrangements of the Gating Ring of the Human BK Channel

Miranda, Pablo, Contreras, Jorge, Wesch, Diana L., Sigworth, Fred J., Holmgren, Miguel, Giraldez, Teresa

Journal:

Biophysical Journal

Année:

2011

Langue:

english

Fichier:

PDF, 39 KB

english, 2011

Multiple gating modes and the effect of modulating factors on the μI sodium channel

Zhou, Jiuying, Potts, Jerald F., Trimmer, James S., Agnew, William S., Sigworth, Fred J.

Journal:

Neuron

Année:

1991

Langue:

english

Fichier:

PDF, 1.12 MB

english, 1991

Imaged by Cryo-EM, Activated and Desensitized GluA2 Glutamate Receptors Show Extreme Flexibility

Shigematsu, Hideki, Yang, Youshan, Yan, Yangyang, Duerr, Katharina, Gouaux, Eric, Sigworth, Fred J.

Journal:

Biophysical Journal

Année:

2014

Langue:

english

Fichier:

PDF, 41 KB

english, 2014

Cryo-EM Structure of Functional BK Channels in Lipid Bilayers

Wang, Liguo, Sigworth, Fred

Journal:

Biophysical Journal

Année:

2009

Langue:

english

Fichier:

PDF, 89 KB

english, 2009

μl Na+ channels expressed transiently in human embryonic kidney cells: Biochemical and biophysical properties

Ukomadu, Chinweike, Zhou, Jiuying, Sigworth, Fred J., Agnew, William S.

Journal:

Neuron

Année:

1992

Langue:

english

Fichier:

PDF, 9.49 MB

english, 1992

Real-Time Detection of the Surface Delivery of Newly Synthesized Membrane Proteins

Jacopo S. Andreose, Guido Fumagalli, Fred J. Sigworth and Michael J. Caplan

Journal:

Proceedings of the National Academy of Sciences

Année:

1996

Langue:

english

Fichier:

PDF, 1.16 MB

english, 1996

[IEEE 2006 IEEE/NLM Life Science Systems and Applications Workshop - Bethesda, MD (2006.7.13-2006.7.13)] 2006 IEEE/NLM Life Science Systems and Applications Workshop - An integrated Silicon-on-sapphire Patch-clamp amplifier

Culurciello, Eugenio, Laiwalla, Farah, Fu, Zhenging, Klemic, Kathryn, Sigworth, Fred

Année:

2006

Langue:

english

Fichier:

PDF, 2.31 MB

english, 2006

Quantifying the local resolution of cryo-EM density maps

Kucukelbir, Alp, Sigworth, Fred J, Tagare, Hemant D

Journal:

Nature Methods

Année:

2013

Langue:

english

Fichier:

PDF, 710 KB

english, 2013

Microfluidic System for Planar Patch Clamp Electrode Arrays

Li, Xiaohui, Klemic, Kathryn G., Reed, Mark A., Sigworth, Fred J.

Journal:

Nano Letters

Année:

2006

Langue:

english

Fichier:

PDF, 181 KB

english, 2006